Gene Identification at the Insertion Site of a High Salinity Tolerant Insertional Mutant Rice Line

Anky Zannati
Utut Widyastuti
Satya Nugroho
June 4, 2018  •  DOI: 10.21082/ip.v27n1.2018.p9-14


High salinity is one of the major abiotic stresses affecting rice production. Previously, we have identified transposonDs insertional mutant rice lines cv Nipponbare showing improved tolerant under salinity stress The objective of thisresearch was to determine the Ds insertion site in chromosomal DNA from one of the salt tolerant mutant line (170-10) and identify the genes in the proximity. A specific amplicon around 450bp from the mutant rice line 170-10 wassuccessfully isolated with TAIL PCR technique using the degenerate and a specific Ds primers. Bioinformatics analysesfound that the insertion was located in a putative CDS designated as Os11g0686500 in chromosome 11 at the 2nd exon.Two putative CDS's within the proximity of the Ds insertion site downstream and upstream the insertion sites were alsoidentified.

Download PDF Cite

Related Journals

Pemanfaatan Model Proyeksi Iklim dan Simulasi Tanaman dalam Penguatan Adaptasi Sistem Pertanian Padi terhadap Penurunan Produktivitas Akibat Perubahan Iklim: Studi Kasus di Kabupaten Sumedang, Jawa Barat

Sistem Pakar Identifikasi Gangguan Organisme Pengganggu Tanaman dan Defisiensi Hara Tanaman Hias Krisan

Aplikasi Metode Tren Waktu Satu Ragam dalam Peramalan Toleransi Komoditas Pangan terhadap Perubahan Iklim di Papua


Search Research and Publications

CARI TULISAN is a scientific publication indexing site that helps everyone find research results and relevant data from papers, journals, books, research reports, and so on. Collected from various repositories, it makes scattered scientific research easily searchable.
All articles and content on this site are copyrighted works of the relevant authors that have been published as a result of scientific research. CARI TULISAN never distributes and supports pirated content.